Spectrophotometry/Standard Curve

Part 1 of 10


This activity tests your mastery of the lab procedure to determine velocity in an enzyme mediated reaction by using a standard curve for the product of the reaction.

Assume that you are studying the following reaction, catalyzed by the enzyme turnip peroxidase:

4 guaiacol + 2 H2O2 tetraguaiacol + 8 H2O

Solution of guaiacol are colorless; solutions of tetraguaiacol are brown. All solutions are made up in a buffer that maintains pH at 7.1, optimum for turnip peroxidase.

From the following list of molecules, select the substrate for this reaction. Press the "OK" button to submit yor answer.

guaiacol
H2O2
tetraguaiacol
H2O
buffer