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Understand what plasmids are and how they are used as vectors for inserting genes into bacteria.

• Question 1
• Question 2
• Question 3

Understand how restriction endonucleases are used to create recombinant DNA molecules and how these enzymes are named.

• Question 1
• Question 2
• Question 3

Understand the importance of methylase enzymes.

• Question 1

Understand how the principles of electrophoresis can be used to separate DNA molecules of different sizes.

• Question 1

Construct a standard curve from size standards run in an electrophoresis gel and estimate the sizes of unknown DNA fragments using a standard curve.

• Question 1

Construct a restriction map based on DNA fragment data from a gel.

• Question 1
• Question 2
• Question 3
• Question 4

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All contents © 2003 Cornell University. All rights reserved.

Prepared by the BioG 1101-1104 Course Staff.
Comments to Introductory Biology: biology_1101@cornell.edu

Revised: August 21, 2008
URL: http://BioG-1101-1104.bio.cornell.edu