Plasmids -- Question 3

You are interested in using the plasmid  pGEE as a vector for incorporating foreign DNA into  E. coli where it can be cloned. The following plasmid map shows the locations of the recognition sequence (cut) sites of five different restriction endonucleases, as well as the ORI and a kanamycin-resistance gene (kan^r ). Click appropriate buttons below to indicate which pair of restriction endonucleases you would use to digest the plasmid and the foreign DNA. Note: several combinations may be correct. Try to find all of them.

First Choice Second Choice BglII BamHI EcoRI SmaI EcoRII BglII BamHI EcoRI SmaI EcoRII