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Bacterial Genetics/Recombinant DNA
Understand what plasmids are and how they are used as vectors for inserting genes into bacteria.
Understand how restriction endonucleases are used to create recombinant DNA molecules and how these enzymes are named.
Understand the importance of methylase enzymes.
Understand how the principles of electrophoresis can be used to separate DNA molecules of different sizes.
Construct a standard curve from size standards run in an electrophoresis gel and estimate the sizes of unknown DNA fragments using a standard curve.
Construct a restriction map based on DNA fragment data from a gel.
visitors to this page since 9/24/97
Prepared by the BioG 101-104 Course Staff.
Comments to Introductory Biology: biology_101@cornell.edu
All contents © 2003 Cornell University. All rights reserved.
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Revised: February 20, 2008
URL: http://BioG-101-104.bio.cornell.edu
