Neurotransmitters—Effects on the Frog Heart


Instructions: each of the following QuickTime® video clips shows a frog heart either without application of a neurotransmitter (control), or after the application of either acetylcholine or epinephrine. Several thirty (30) second timed intervals are indicated with audio signals so you can determine the heartbeat rate (beats/30 sec). Compare the data for the control with data collected after application of acetylcholine or epinephrine to determine the effects of these two neurotransmitters on the frog heart. Note: this tutorial requires that the QuickTime 5 plugin is installed in your browser.

Treatment

Video Sequence

Control—two 30-sec. timed intervals for a heart without any applied neurotransmitters.

Epinephrine—four 30-sec. timed intervals after application of 4 drops of 5 X 10-5 M epinephrine.

Question: does the heart beat more forcefully than in the control?

Acetylcholine—four 30-sec. timed intervals after application of 2 drops of 5 X 10-4 M.

Question: do you see any irregular heartbeats?



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Prepared by the BioG 101-104 Course Staff.
Comments to Introductory Biology: biology_101@cornell.edu

All contents © 2003 Cornell University. All rights reserved.
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Revised: November 15, 2007
URL: http://BioG-101-104.bio.cornell.edu